IN THIS EXPERIMENT YOU WILL…
Perform an experiment to determine the sensitivity of enzymes to temperature changes.
The box includes:
- Experiment Booklet
- Digital Software
- 3 x 400 ml glass beakers
- 3 x petri dishes
- Thermometer 150mm (-10/110˚C)
- Measuring cylinder 100ml
- Glass rod (Stirring Rod)
- Spoon end Spatula
- Biological washing powder (Must have red and blue particles)
- 3 pieces of white cotton cloth – 5cm x 5 cm
- Eosin Y Stain 10ml
- Bromothymol Blue stain 10ml
- Food colourant 10ml
To be supplied by Educator
- Kettle with boiled water
(Optional to use Gauze Wire and Burner if the teacher has no access to a kettle)
- Markers – to mark the different beakers
- Tap water
- Ice water
- Burner and Fuel cartridge
- Gauze wire ceramic
HOW DOES IT WORK?
Time allocation: 45 Min
Enzymes are biologically important chemicals produced to breakdown macro-particles such as proteins into smaller molecules in a process known as metabolism. The enzymes latch on to a molecule (in this case a protein) and with the help of a catalyst, in a process called the Lock and key process, the protein is broken down into various molecules that are used by the body to build important tissues.
The Biological enzyme washing powder demonstrates this in this experiment. This technique is used by forensic anthropologists, palaeontologists and other scientists to clean biological compounds off bone samples. In the experiment the reduction in staining of the cloth is reliant on the presence of the biological enzyme washing powder. The boiling water degraded the enzymes in the washing powder, meaning that the enzymes will not be as effective.
YOU SHOULD KNOW…
- Enzymes are proteins
- Enzymes are specific in their reactions
- Enzymes are sensitive to temperature changes
- Enzymes are sensitive to pH changes
- Enzymes are biological catalysts – that lower the energy necessary for metabolic processes.
- Do not rub the cloth during the rinsing process.
- Stains must be the same on all three pieces of cloth.
- Make sure to follow instructions precisely to ensure accurate results.
- Workstations must always be kept need and tidy.